Молекулярное клонирование

Molecular cloning

Mamatova Irodakhon

Молекулярное клонирование

Cloning steps

Choice of host organism and cloning vector
Preparation of vector DNA
Preparation of DNA to be cloned
Creation of recombinant DNA with DNA ligase
Introduction of recombinant DNA into host organism
Selection of organisms containing vector sequences
Screening for clones with desired DNA inserts and biological properties

Молекулярное клонирование

Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms

Молекулярное клонирование

E. coli and plasmid vectors are in common use because they are technically sophisticated, versatile, widely available, and offer rapid growth of recombinant organisms with minimal equipment

Молекулярное клонирование

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20.01.2020